Effect on postoperative visual quality with modified ABC design aspheric IOL implantation of cataract patients / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To evaluate the visual quality of patients after modified design aspheric balance curve(ABC)with intraocular lens(IOL)implantation, and to analyze the influencing factors of clinical IOL selection and guide the patient's IOL selection plan. METHODS: A prospective case-control study was conducted in 67 patients(74 eyes)with simple cataract underwent phacoemulsification and foldable aspheric IOL implantation, and 23 eyes in the observation group were implanted with modified design IOL(HOYA Vivinex XY1 group), the control group was implanted with 51 eyes of traditional design IOL(Tecnis ZCB00 group with 27 eyes, IQ SN60WF group with 24 eyes). The uncorrected visual acuity, the best corrected visual acuity, total ocular spherical aberration(SA)and coma under different pupil diameters(3, 4, 5, 6mm), and different pupil diameters(3, 4, 5mm)were measured 1wk and 1mo after operation, the modulation transfer function(MTF)curve, objective scattering index(OSI), intraocular scattered light value Log(s)and contrast sensitivity were obtained. Statistical analysis was performed on the obtained data.RESULTS: The uncorrected visual acuity and best corrected visual acuity at 1wk and 1mo after operation in the three groups were significantly improved compared with those before operation, there was no significant difference among groups(P>0.05). The difference of total ocular spherical aberration was statistically significant among the three groups with 5 and 6mm pupil diameter 1wk after operation(P=0.045, 0.037)and there were differences among three groups in pupil diameter of 6mm at 1mo after operation(P=0.042). Comparing the total ocular coma aberration, there were differences among the three groups at 1wk and 1mo after the operation at the pupil diameter of 5 and 6 mm(P<0.05). With the increase of pupil diameter at 1wk and 1mo after operation, the total ocular spherical aberration in the HOYA Vivinex XY1 group was lower than that in the other two groups. The MTF values of the Vivinex XY1 group were higher than those that of the control group at each spatial frequency, there was no significant difference among groups(P>0.05), and there were no statistical differences in objective scattering index, intraocular scattered light value Log(s)and contrast sensitivity among the three groups(P>0.05).CONCLUSION:The improved design of the modified Vivinex IOL can reduce the total ocular spherical aberration and coma, improve the visual quality, and provide a new method for the selection of aspheric IOL.

3, 4-seco-Labdane diterpenoids from the leaves of Callicarpa nudiflora with anti-inflammatory effects / 中国天然药物

Four new 3, 4-seco-labdane diterpenoids, nudiflopenes J-M, were isolated from the leaves of Callicarpa nudiflora along with six known compounds. The structures of these diterpenoids were determined by comprehensive spectroscopic analysis. All the isolated compounds were evaluated for their inhibitory effects on NO production in LPS-stimulated RPMs and RAW264.7 cells. The results suggest that nudiflopenes J-M and other four known compounds showed significant inhibitory effects against NO production comparable to the positive control dexamethasone.

Effect of exogenous hydrogen sulfide on expression of NLRP3 inflamma-some in hepatocytes / 中国病理生理杂志

AIM:To evaluate the effect of exogenous hydrogen sulfide(H2S)on the expression of NLRP3 in-flammasome in hepatocytes.METHODS:The hepatocytes L 02 and SMMC-7721 were used to establish the model of inflam-mation by stimulating with lipopolysaccharide(LPS)at different concentrations in vitro.The expression of NLRP3 inflam-masome in the hepatocytes was detected by Western blot and the cell viability was measured by MTT assay for determining appropriate concentration of LPS.The hepatocytes were divided into 4 groups:the cells in control group were incubated with normal medium for 18.5 h;the cells in LPS group were incubated with normal medium for 0.5 h followed by 100 μg/L LPS for 18 h;the cells in LPS+H2 S group and H 2 S group were incubated with 200μmol/L sodium hydrosulfide hydrate(NaHS)for 0.5 h followed by 100 μg/L LPS or normal medium for 18 h,respectively.The protein expression of NLRP3 and caspase-1 in the cells of every group was determined by Western blot.RESULTS:Compared with control group ,the protein expression of NLRP3 and caspase-1 increased significantly in LPS group(P<0.05)and had no significant change in H2S group.Compared with LPS group,the protein expression of NLRP3 and caspase-1 in LPS+H2S group decreased significantly(P<0.05).CONCLUSION:In hepatocytes,exogenous H2S suppresses the expression of NLRP3 inflamma-some.

A construction of training tasks about preventive maintenance for medical devices by multi-factor model and analytic hierarchy process / 中国医学装备

Objective: To construct the training tasks about preventive maintenance for medical devices based on multi-factor model and analytic hierarchy process(AHP). Methods: The preventive maintenance items of 8 general medical devices were analyzed in the research. Through carried out multi-factor model and analytic hierarchy process to design reasonable training subjects for preventive maintenance of medical devices, and through combined with decision-making tree model of criticality difficulty frequency (CDF) to implement the contrast with judgment standard. Multi-factor model set 2.5 score as the standard score of professional training subjects, and subject need carry out professional training if score ≥2.5, otherwise it needn't. Results: In the grading of 8 subjects of multi-factor model, the scores of 5 subjects were more than 2.5 score, and they need implement professional training. This result was coincidence with the analysis result of decision-tree model of CDF. Conclusion: The analysis method of multi-factor model and analytic hierarchy process has important value for setting reasonable training subject for preventive maintenance of medical device, and it can provide theoretical basis for setting standardized training subject in future.

Chemical constituents of whole plant of Euphorbia thymifolia / 中草药

Objective: To separate and identify the chemical constituents from the whole plant of Euphorbia thymifolia. Methods: The constituents were isolated and purified by various chromatographic methods and were identified on the basis of spectral analysis and physicochemical characters. Results: Thirteen compounds were separated and identified as luteolin (1), apigenin (2), quercetin (3), kaempferol (4), ethyl galloyl acid (5), p-coumaric acid (6), protocatechuic acid (7), gallic acid (8), caffeic acid (9), 3, 4-seco-8βH-ferna-4 (23), 9 (11)-dien-3-oic acid (10), 3, 4-seco-oleana-4 (23), 18(19)-dien-3-oic acid (11), tricosanol (12), and β-sitosterol (13), respectively. Conclusion: Addition to the compound 5, the other compounds are isolated from this plant for the first time.

Pressure therapy upregulates matrix metalloproteinase expression and downregulates collagen expression in hypertrophic scar tissue / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Pressure therapy improves hypertrophic scar healing, but the mechanisms for this process are not well understood. We sought to investigate the differential expression of matrix metalloproteinases (Mmps) and collagen in posttraumatic hypertrophic scar tissue with mechanical pressure and delineate the molecular mechanisms of pressure therapy for hypertrophic scars.</p><p><b>METHODS</b>Fibroblast lines of normal skin and scar tissue were established and a mechanical pressure system was devised to simulate pressure therapy. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting assays were used to compare differences in the mRNA and protein expression of Mmps and collagen in scar fibroblasts before and after pressure therapy.</p><p><b>RESULTS</b>The expression differed between the hypertrophic scar cell line and the normal cell line. RT-PCR assays showed that Collagen I, highly expressed in the hypertrophic scar cell line, decreased significantly after pressure therapy. Mmp2, Mmp9, and Mmp12 expression in the hypertrophic scar tissue increased significantly after pressure therapy (P < 0.05). Western blotting assays further revealed that Mmp9 and Mmp12 expression increased significantly in the hypertrophic scar tissue after pressure therapy (P < 0.05) but not Mmp2 expression (P > 0.05).</p><p><b>CONCLUSION</b>Mechanical pressure induces degradation of Collagen I in hypertrophic scar tissue by affecting the expression of Mmp9 and Mmp12.</p>

Impact of disease activity on postoperative recurrence and complications after bowel resection for Crohn's disease / 中华外科杂志

<p><b>OBJECTIVE</b>To determine whether the perioperative disease activity is associated with recurrence and complications after bowel resection for Crohn's disease (CD).</p><p><b>METHODS</b>Clinical data of patients underwent bowel resection for CD at the Nanjing General Hospital of Nanjing Military Command from January 2002 to January 2011 was retrospectively analyzed. Postoperative recurrence and complications in patients with active disease were compared with those in patients with remission.</p><p><b>RESULTS</b>A total of 90 patients underwent bowel resection for CD, active disease were seen in 43 patients at the time of surgery, while the rest 47 patients were in remission. The postoperative cumulative endoscopic recurrence rate was 8.5% at 1 year, 27.7% at 2 years and 44.7% at 3 years in the patients with remission, and was 27.9% at 1 year, 37.2% at 2 years and 53.5% at 3 years in patients with active disease. Data indicated the endoscopic recurrence were statistically significant in the first year after surgery (χ² = 4.605, P = 0.032). Additional, the postoperative complication rates in patients with remission (14.9%) was significantly lower than that in patients with active disease (51.2%) (χ² = 6.979, P < 0.001).</p><p><b>CONCLUSION</b>Patients with active disease at the time of surgery were encountered with early postoperative recurrence and increased complications after intestinal resection for CD.</p>

Aesthetic fingertip reconstruction with partial second toe transfer / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Fingertip defect significantly affects the appearance of the hand. The aim of this research was to evaluate the clinical effect of aesthetic fingertip reconstruction with partial second toe transfer.</p><p><b>METHODS</b>Between July 2005 and December 2008, 17 patients underwent aesthetic fingertip reconstruction with partial second toe transfer. The mean size of fingertip defects was 0.7 cm (ranging from 0.5 - 1.1 cm). Anastomosis was performed between one dominant plantar digital artery and one proper digital artery, between two dorsal digital veins of the toe and two dorsal digital veins of the finger, respectively, for the reconstruction of blood supply to the reconstructed finger. Two plantar digital nerves of the toe were then sutured to two proper digital nerves of the finger for the restoration of fingertip sensation.</p><p><b>RESULTS</b>All reconstructed fingers survived completely. The follow-up period ranged from 6 to 24 months, and the appearance of the reconstructed fingertip was similar to that of the normal fingertip. Two-point discrimination of the reconstructed fingertip was (7.8 ± 1.3) mm. The recovered pinch strength of the reconstructed finger was about (89.0 ± 5.1)% of that of the normal finger. Donor site healed well without complications.</p><p><b>CONCLUSIONS</b>Partial second toe transfer is an ideal aesthetic reconstruction method for fingertip defects; it can not only achieve a satisfactory appearance of the fingertip, but can also obtain excellent sensory and motor functions.</p>

Reconstruction of soft tissue defect of the extremity with the perforator flap from inguinal region / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The perforator flap has become a very useful reconstructive means of soft tissue defect of extremities. The perforator flap from the inguinal region has advantages that include the ability to cover a large area with little donor site morbidity and provision of suitable thickness for reconstruction.</p><p><b>METHODS</b>From July 1, 2005 to June 30, 2007, 10 patients with various types of soft tissue defect underwent reconstruction with 10 perforator flaps from the inguinal region. Six flaps were applied to the upper extremities and four flaps to the lower extremities. The dimensions of the transferred flaps ranged from 7-30 cm in length and 4-10 cm in width.</p><p><b>RESULTS</b>The postoperative course of all 10 flaps was uneventful and all flaps survived. Distal small partial necrosis was observed in one case due to arterial insufficiency of the distal part of the flap. All patients were satisfied with the functional and esthetic resurfacing outcome.</p><p><b>CONCLUSION</b>Use of perforator flap from the inguinal region could overcome the disadvantages of the traditional free groin flap, and represents a safe and useful tool for coverage of soft tissue defects.</p>

An alternative approach in the treatment of thumb web contracture skin defects: lateral tarsal artery flap / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Thumb web contracture is a common complication after hand injury, and can markedly affect whole hand function. Therefore, surgery involving thumb web reconstruction is often necessary to restore normal function of the involved hand. In this study, we present the application of the lateral tarsal artery (LTA) flap in first web reconstruction.</p><p><b>METHODS</b>From November 1, 2005 to October 31, 2007, seven patients with severe post trauma or burn contractures around the first web space were treated with a LTA flap. All the patients were followed up.</p><p><b>RESULTS</b>All flaps survived, with an average size of 6.7 cm x 4.8 cm. There were no complications or recurrent contractures during follow-up. All patients were satisfied with the esthetic appearance and functional outcome of the reconstruction.</p><p><b>CONCLUSION</b>The LTA flap could be an excellent option for covering various defects in the thumb web space, serving as an excellent alternative for the thumb web space reconstruction.</p>

Effects of Arbuscular Mycorrhizae on growth and essential oil of Atractylodes lancea / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of Arbuscular Mycorrhizae on cultivated Atractylodes lancea.</p><p><b>METHOD</b>Pot experiment of A. lancea, with (code as AM) or without (code as CK) Glomus mosseae (GM) was conducted 5 times respectively, then the biomass, essential oil, and soil nutrition, soil organism, soil microbial were detected after A. lancea were harvested.</p><p><b>RESULT</b>(1) Mycorrhizal dependency of A. lancea was 245%, and height of individuals, numbers of leaves, leaf area, biomass of A. lancea were all higher in AM than in CK (P < 0.05). (2) GC-MS analysis with cluster analysis and principal components analysis showed that there were no differences in essential oil of A. lancea between AM and CK. (T3) Total N, available N, available P and available K in AM soil were all lower than in CK soil. (4) GC-MS analysis showed organic matters changed differenly in AM soil and CK soil, components 5,6 in AM soil were higher than that in CK soil, but component 9, 10, 11 were lower in AM soil than that in CK soil. (5) Biolog detect showed AWCD of AM soil microbe were higher than that of CK soil throughout the incubation, and AWCD of the former was 0.66, and the later was 0.46 after 192 h incubation. and t-test showed, Shannon seven indices and McIntosh'seven indices were same both at 72 h and 192 h, and diversity indices of Shannon and McIntosh were also same at 72 h, but AM soil microbe were higher than CK soil microbe at 168 h (P < 0.05).</p><p><b>CONCLUSION</b>AM could promote nutrition uptake, improve the function diversity and activity of microbe in rhizosphere of A. lancea, influence the composition of the organic matter, that lead the growth of A. lancea, but not to the quality.</p>

Transgenic wheat (Triticum aestivum L.) with increased resistance to the storage pest obtained by Agrobacterium tumefaciens--mediated / 生物工程学报

The transgenic wheat of improved resistance to the storage pest was production. We have introduced the cowpea trypsin inhibitor gene (CpTI) into cultured embryonic callus cells of immature embryos of wheat elite line by Agrobacterium-mediated method. Independent plantlets were obtained from the kanamycin-resistant calli after screening. PCR and real time PCR analysis, PCR-Southern and Southern blot hybridization indicated that there were 3 transgenic plants viz. transformed- I, II and III (T- I, T-II and T-III). The transformation frequencies were obviously affected by Agrobacterium concentration, the infection duration and transformation treatment. The segregations of CpTI in the transgenic wheat progenies were not easily to be elucidated, and some transgenic wheat lines (T- I and T-III) showed Mendelian segregations. The determinations of insect resistance to the stored grain insect of wheat viz. the grain moth (Sitotroga cerealella Olivier) indicated that the 3 transgenic wheat progeny seeds moth-resistance was improved significantly. The seed moth-eaten ratio of T- I, T-II, T-III and nontransformed control was 19.8%, 21.9%, 32.9% and 58.3% respectively. 3 transgenic wheat T1 PCR-positive plants revealed that the 3 transgenic lines had excellent agronomic traits. They supplied good germplasm resource of insect-resistance for wheat genetic improvement.